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A RSM was fit to data on the maximum biomass (K, estimated from each of the three replicate time series).
A RSM was fit to data on the maximum biomass (K, estimated from each of the three replicate time series) and TAG concentrations were calculated, per cell, during the stationary phase to assess biomass and TAG accumulation over time as a function of NH4Cl and initial WT:mutant cell number ratio.
Data shown was obtained from two biological replicate time courses.
Three plants were combined from each replicate time sample.
Replicate time courses were centred around their common mean to remove systematic shifts in the intensities.
To investigate P. falciparum lncRNA transcription, we harvested fifteen blood stage samples from two biological replicate time courses [Fig. 1A].
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First, that at least two replicate time-series measurements have been produced.
Variability was further reduced by combining slices from each cut within a group, and performing replicate time-course studies.
This method, which requires replicate time-series experiments, is based on a statistical framework and uses polynomial regression to account for the temporal nature of the data.
However, as the kinetics of release from synchronization always varies from experiment to experiment [ 4], replicate time-series experiments will not progress through the cell-cycle in exactly the same way.
Though many people viewed the book's assertions as counterintuitive, the social science evidence has been replicated time and again.
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