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Following replicate testing (three independent experiments in quadruplicate), mean ± SEM O/E values were calculated and the significance (p-values) for O/E reductions in MCF7/HER2 or BT474 relative to MCF7 cells were determined by ANOVA and t testing.
IHC results for HER2/neu vary with replicate testing using the same antibody clone and when alternate clones are utilized.
In the three specimens with detectable sequences, we noted that the copy number was very low as nested PCR and replicate testing was often needed for detection.
The nested gag RT-PCR assay had a sensitivity between 25 and 100 copies per reaction in replicate testing of XMRV RNA 92.5% (37/40) and 97.5% (39/40), respectively, but could also detect 5 copies in 50% (20/40) of the replicates.
Considering the present work a pilot study, replicate testing of the samples was not performed.
Despite this, the PCR/ESI-MS and culture methods both showed similar agreement on replicate testing using the κ statistic.
Similar(45)
Nonetheless, if our results are replicated, testing for FMc could be used to identify healthy women who might be at risk for developing breast cancer.
Clear justification and selection criteria should accompany any focal species strategy to allow methods to be replicated, tested, and refined.
The proposed framework, which can be replicated, tested, and evaluated in empirical research, will add additional depth to studies in the field of smart sustainable cities.
Intra-day precision was determined from the analysis performed on the same day of eight independent replicates at low, medium and high levels of nicotine and inter-day precision was calculated on five independent replicates tested over five days.
Increasing the number of assay replicates tested resulted in high sensitivity for CRC with excellent specificity in healthy controls.
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