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Owing to the large number of mice needed for this study (48 in total), the experiment was carried out in two replicate steps, each of which involved the training and testing of 6 mice from each experimental group (WT-Hom, KO-Hom, WT-Het, KO-Het).
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Wine & Palette in Oklahoma City regularly holds painting events in restaurants and wineries, enlisting the help of a professional artist to create a painting, which participants then replicate step by step with a wine glass in hand.
Hoops et al. [ 57] tried but unfortunately could not replicate the step-up and step-down illumination experiments with Volvox rousseletii and Volvox capensis, both of which belong to the section Volvox.
A model was built in Microsoft Excel to replicate pathway steps and estimate costs.
This large number prohibited use of the manual procedure detailed in Section 4.2(3–5) and an automated procedure was developed to replicate these steps.
FFPE cell lines were processed in the same manner as FFPE tissue (microdissection and Liquid Tissue preparation) in order to replicate the steps involved in SRM analysis of FFPE tumor tissue.
Other techniques, "pearl growing" and "snowballing" where one relevant item is used to obtain others can also be used in systematic reviews [ 6] although these are not always described in sufficient detail to be able to replicate the steps in the process.
A separate RIKEN team is now trying to replicate every step of the experiments.
In a related development, at a press conference on Monday, Hitoshi Niwa, a RIKEN senior scientist and a co-author of the papers, outlined how a RIKEN team will try to replicate every step of the experiments reported in the two papers, from the stressing of the cells and the creation of STAP stem cells to testing their pluripotency.
During expansion of latency in B cells, viral genomes replicate in step with normal cell division.
This program uses a Bayesian MCMC search strategy of a single, replicated, two million step chain.
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