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A minimum of three replicate slices were used for each brain per experiment.
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Replicate ovary slices were removed, snap frozen in liquid nitrogen, and stored at -80°C until needed for RNA extraction.
Surface radioactivity (counts per minute per mm) was measured and averaged from three replicate brain slices from each rat using Beta-vision plus software (Biospace Labs).
Before protein digestion, gel lanes for each biological replicate were sliced into 10 equal size segments, diced into approximately 1 mm cubes with a clean scalpel and transferred into a 96 well plate device (MultiScreen Solvinert Plates, Millipore).
Each floor replicates the slice of ground beneath it.
The trabecular bone was separated from the cortical bone by manually drawing a contour in the proximal tibia while, in the distal femur, an elliptical region of interest (length/width ratio of 1.5) was drawn and replicated every slice.
At each station, three replicate samples were sliced per cm down to 5 cm sediment depth and fixed in buffered 4% formalin.
For each exposure, ovaries from 6 replicate fish were sliced into 12 ± 5 mg pieces and randomly distributed across sample culture wells to minimize sample effects due to potential tissue heterogeneity.
Replicate 5-μm-thick slices were cut from frozen tissue blocks.
For stable isotope analysis of nematodes, sediment from within each replicate, but from several slices was combined in 0 2 cm, 2 5 cm, 5 8 cm and 8-end cm to gather sufficient nematode biomass.
MV replicated optimally in lung slices of macaques, HMPV and HRSV in lung slices of cotton rats, and CDV in lung slices obtained from dogs and ferrets.
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CEO of Professional Science Editing for Scientists @ prosciediting.com