Sentence examples for replicate sequence from inspiring English sources
For each set of conditions, 500 replicate sequence alignments were generated.
28 PCR errors may lead to replicate sequence artifacts, which can cause overestimation of species abundance and functional gene abundance in 16S rRNA and full shotgun metagenomics, respectively.
In each replicate sequence data for 4000 base haplotypes for each of 30 chromosomes was simulated using the MaCS (Chen et al. 2009).
The distributions of estimated rate differences between ingroup taxa among replicate sequence simulations for all six different nucleotide substitution model parameter sets (see Table 2) are shown in Figure 3.
Discordance may refer to an insertion, a deletion, a nucleotide mismatch or an ambiguous base call (N) with respect to a non-available nucleotide determination on the replicate sequence (according to Huse et al. [ 10]).
After rejecting variants resulting from potential sequencing errors, including under-reads of homopolymer regions, and single base insertions, the variants were confined to those confirmed in at least 90% of replicate sequence reads.
Error bars show standard error for replicate sequencing.
Three biological replicates of each treatment were analysed, with each biological replicate sequenced twice in separate lanes (sequencing replicate).
Replicate sequences for each sample were aligned and a consensus sequence was obtained.
First, the use of replicate sequencing increases costs and necessitates additional input nucleic acid amounts.
Thus, we performed replicate sequencing runs for each of the PGM and MiSeq libraries.
