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Thirteen infection cycles were performed for each of 11 replicate selection lines in each treatment.
We have generated replicate selection lines of house mice evolving with and without sperm competition.
Hurd et al. [ 13] created three replicate selection experiments, referred to as the black, red and green groups.
Each replicate selection line provides one estimate of the realized heritability, allowing a mean and standard error to be directly estimated for each selection treatment.
We formed two replicate populations by a random division of flies in the IV population, then founded three treatments in each replicate: selection up, selection down, and a control.
However, it is striking that the mean sperm quality scores from generation eight [ 12] follow the rank order of mean P2 values found here across the replicate selection lines.
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Samples from at least two independent replicate selections were hybridized for each antibiotic.
This is, in part, owing to the fact that selection studies are rarely replicated either temporally or spatially [ 41] and when studies are replicated, selection estimates tend to be highly variable among replicates [ 68].
We reared individuals in each generation in identical environmental (Supporting Information S1) and density conditions in three replicated selection treatments similar to Conover and Munch's (2002) landmark study.
The guidelines described internationally for performing agronomic and phenotypic analyses of GM crops emphasize that choosing appropriate comparators and performing adequate field trials (e.g., number of growing seasons, replicates, selection of characteristics to be analyzed) are crucial to ensure confidence in the results.
The null hypothesis of no karyotype effect was tested here after performing random permutations among replicate and selection temperature for the among selection temperature F-statistics.
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