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Gardner et al [ 20] evaluated the extent to which a multidimensional adaptive test could be used to replicate screening decisions based on the Pediatric Symptom Checklist.
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The relatively small overlap between these apparently biologically similar experiments is unexpected given that the principal differences between the SRSF and HFA screens are the libraries used and the number of replicates screened.
Using this approach and 5 replicate screens, we identified 7 extracts that reproducibly changed insulin and/or pdx1 promoter activity from a library of 1319 marine invertebrate extracts.
We present results from multiple replicate screens that highlight several extracts capable of significantly altering insulin and pdx1 promoter activity in beta-cells.
In total, we used data from three biological replicate screens in the final analysis.
These screens were performed on all eight libraries, including replicate screens for one library of each cell line.
Note that normalization steps result in the same number of node hits for replicate screens shown in Figure 1.
While replicate screens ideally should result in identical hit lists, experimental variability makes this not the case.
Each of the replicate screens contained five 384-well plates seeded with cells and incubated with 306 drugs and controls.
First, replicate screens of each cell line showed good correlation; the average pair-wise Pearson correlation coefficients (R-values) for cell line replicates ranged from 0.3-0.9 0.3-0.9.5).
In contrast, replicate screens (screens performed using the same protocols and siRNA set, but performed at a different time) were reproducible (R was between 0.5 0.9).
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