The median rs from the 10,000 replicates reflects the correlation based on the median pairwise diversity and the confidence interval obtained from the distribution of the statistic for the resampled data reflects the underlying variance in the estimate of pairwise diversity.
The variability of the point estimate across partitions (replicates) reflects uncertainty due to sampling of TRN and TST sets, and a precise estimate of prediction accuracy can be obtained by averaging the estimates of accuracy obtained in each partition.
Following rarefaction analysis to confirm that the number of trees sampled per transect was adequate (using EcoSim7.72 [38], [38]), we decided to analyse all interaction networks separately (i.e. considering transect-based networks as within-patch replicates reflecting spatial variation in the composition of tree-epiphyte communities).
Finally, the positive bias found in the peer results at pre-test were not replicated, reflecting that this was probably caused by concerns about confidentiality.
This was also the case for sample B (100% HBRR) and D (25% UHRR, 75% HBRR) replicates, reflecting their relative biological similarity (Additional file 1A).
The number of genes mapped in each tissue was consistent between biological replicates, reflecting the robustness of our library preparation from tissue-specific RNA samples.
Hybridization statistics were used to determine a two-fold change in expression, consistent across all replicates, reflected a statistically significant (p values and standard errors generated by analysis not shown) difference in expression between genotype and iron concentrations.
Furthermore, the choice of studying 100 replicate populations reflects the 100- or 96-well plate experimental setup that is commonly used (Lemonnier et al. 2008; Kvitek and Sherlock 2011).
The data, representative of three biological replicates, consistently reflects augmented accumulation of p52 protein in the costimulation regime as compared to cell treatment with LPS or αLTβR alone for 24h.
In summary, the present data show that even in an ultra-short screening version of the EMBU and its German version, FEE, the factorial structure could be replicated, which reflects the quality of the instrument for the retrospective assessment of subjective representations of parental rearing behavior.
