Exact(3)
This paper presents a method to derive kinematic requirements that a theoretical simulator should meet to best replicate reference maneuvers within a predefined workspace.
The feature of our experimental design that allows samples to be compared even when control and experimental samples are in completely separate experiments is that identical replicate reference samples of pooled RNA are processed in each batch in every experiment.
The unique feature of our experimental design that enables us to compare data from separate experiments is that identical replicate reference samples of pooled RNA (that are derived from the same tissue as are the experimental samples) are included in each batch within each experiment.
Similar(57)
We applied a so called replicated reference design [ 18]; every treated sample was hybridized to a unique reference (untreated) sample.
For hybridization a so called replicated reference design was used, wherein the 4 low phenanthrene concentration (24.95 mg kg-1) exposed samples and the 4 high phenanthrene concentration (45.80 mg kg-1) exposed samples were mixed with 8 unique non-spiked reference samples.
U-pside: What is the potential to replicate, expand, reference, or create new business from this deal?
The parameters were used as follows: Window size: 200 bps; Step size: 10 bps; Tree algorithm: Neighbor; Distance model: Kimura; Bootstrap replicate: 100; Reference type: 50 % consensus.
Given our limited sample size in each batch as well as the limited number of replicate identical reference samples in each batch, this Bayesian approach is an appropriate choice.
For each replicate in reference sample Dataset 5, the biological specimens for conditions X and Y were prepared on the same day, so a natural pairing exists for the condition X and Y arrays.
This overlap (the common set of mutation parameters needed to replicate both reference phenomena) only occurs in the middle range of mutation DFE variance (Y axis in plots in Fig. 5B) and requires a ≥4-fold mutation rate increase over lifetime (X axis in plots in Fig. 5B).
All microarray analyses presented herein were based on a sample size of 4 (two replicates of Reference 1 and two replicates of Reference 2 for each platform).
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