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Triplicate sample preparations were done on each sample, and two separate HPLC injections were carried out for each replicate preparation.
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The apparent value of the Michaelis Menten constant (K m ) and catalytic constant (k cat) of the purified rPsaDyP were determined for ABTS, DMP, guaiacol and RBlue 5. Substrate turnover took place at 30 °C in 6 replicate preparations.
Two replicate preparations were analyzed for all cell types.
There was no consistent effect across replicate preparations on total protein expression over one hour treatment with either RAD001 or AZD8055.
Replicate preparations using the same method are the next lowest in variability, again with same day hybs being less variable than different day hybs.
Replicate preparations using different aliquots of a single C2C12 RNA sample (500 ng) were used to determine the reproducibility of the reverse transcription process as well as the results of the same reverse transcription products performed on different days.
Each culture was allowed to grow to confluency and then total RNA was extracted from 5 × 106 Drosophila cells from each of the four replicate preparations using the RNeasy Mini Kit (Qiagen), following the manufacturers instructions.
The mean fluorescence of stained nuclei in replicate preparations of Varroa and D. virilis standard was quantified using a Coulter Epics Elite flow cytometer (Coulter Electronic), with excitation provided by a laser tuned at 488 nm and 25 mW.
"As for the racing side of things, I'm sure the individual coaches will arrange time trials if they see fit to replicate that preparation".
At the time when this study was designed and performed, it cost $300 per replicate library preparation (Illumina TruSeq kit; see Additional file 1: Table S6).
The replicate DNA preparations produced identical patterns for each strain in both typing methods.
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