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By using the biological replicate number required in microarray experiments for a given power or by determining the power required to detect a gene significantly differentially expressed, given the sample size, or the best multiple-testing method can be chosen.
Thus, the within-field replicate number per treatment was four totalling eight for the entire study.
Increasing concentrations of the test compounds were added to cells, with a replicate number of three wells per concentration and 17-AAG served as positive control.
This expanded tool has already allowed us to assess potential positional deposition within the exposure chamber and increase replicate number per exposure.
The replicate number for each plants was listed as following: a No. 1 plant: n = 3 for white light (WL), and n = 4 for blue light (BL).
In Box Behnken design, number of experiments is calculated by following equation: {text{N}} = {text{k}}_{ 2} + {text{k}} + {text{cp}} (1 where k is the factor number and cp is the replicate number of the central point (Edrissi et al. 2008).
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Replicate numbers given indicate the number of biological replicates, except for in the case of qPCR sample analysis where in each case two technical replicates were performed of each of three biological replicates.
Moreover, NOECs from two experiments with different concentration patterns and varying replicate numbers can hardly be compared.
The quantitative precision is summarized in Figure 2. Increases in C and RIMS-sample replicate numbers both generally conferred significant precision improvements.
These results suggest that WAD can perform well across a range of replicate numbers.
The biological replicate numbers are indicated on top of each bar and/or as the n numbers in the legends.
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