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The normalized data of each biological replicate is presented for control and drought-stressed plants.
A summary flatfile containing the total raw reads for each gene in each biological replicate is presented in Data File 2. Genes were excluded if no reads occurred in any biological sample.
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Expression levels of the average of three technical replicates of each biological replicate are presented relative to the expression in the pH 7 anaerobic control.
The summary data, including quality scores, are presented in Data File 1, and the raw transcript read counts for each biological replicate are presented in Data File 2. The FASTQ files generated for each biological replicate are available at the Sequence Read Archive (SRA), a freely accessible database provided by NCBI (http://www.ncbi.nlm.nih.gov/sra/), under the accession number PRJNA185305.
Data are presented as mean ± SD for at least three replicate measurements (the number (n) of replicates is presented in each figure caption).
Western blots were quantified using ImageJ, and % degradation from 3 4 independent replicates is presented.
For TAG assays, a representative of four biological replicates is presented.
The mean of three technical replicates is presented for each experimental treatment; all experiments were conducted at least twice.
A simple statistical method for estimating variances from microarray control data which does not require multiple replicates is presented.
Representative western blots, from at least three biological replicates), are presented.
The percentages of replicate trees in which the associated taxa clustered together based on the bootstrap test (100 replicates) are presented next to the branches.
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CEO of Professional Science Editing for Scientists @ prosciediting.com