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We mapped the predicted microRNA target transcripts on the tissue atlas and considered a transcript as expressed in a specific tissue, if either one replicate has a present call or both show at least a marginal call, similar to the work of McClintick et al. [57].
If replicates agree completely (all x i = x), w should be larger than the largest weight wmax among the replicates (since confirming measurements improve the reliability of x), but if an added replicate has a weight much smaller than wmax, its effect on w should be negligible.
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Each replicate was run for 10 000 generations to reach equilibrium, so each simulation replicate has an independent starting condition.
The results of experiment 1 showed that when recipient oocytes in a replicate had a maturation rate of <40% (34 ± 3.0%; three replicates) the proportion of nuclear transferred oocytes that developed to blastocyst was 2 ± 1.1%, which was significantly lower (P < 0.01) than the 25 ± 3.2% achieved when the recipient oocyte maturation rate was 71±3.7%7% (three replicates).
Replicate had a significant impact on HPG development (F5,108 = 6.24, p < 0.001).
Mature neurons, which no longer replicate, have a greater tendency to display aggregates, although there is no correlation between the level of mHtt expression and the rate of cell death in vivo.
All SNPs we were attempting to replicate had an info score of >0.7.
Normalized signals from totally independent replicates have a mean correlation coefficient of 0.963014.
The Agilent arrays we used displayed better correlation between replicate experiments for most barcodes than displayed by the SUBarrays (Supplemental text S1; Figure S5); however, due to the presence of a number of anti-correlated barcodes in these Agilent experiments, the correlation coefficient between dye-swap replicates had a similar value of 0.7 (Table 1).
Technical replicates had a very high reproducibility, with correlation coefficients of 0.92.
All series of measurements (technical replicates) had a standard deviation below 1.5%.
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