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The strategy of congenic breeding was used to replicate detection of the chromosome 4 QTL and to assess its contribution to the infection phenotype.
These findings are controversial, because other laboratories have been unable to replicate detection of MMTV and because the DNA amplified by PCR in some laboratories has had homology with nonviral sequences in the human genome (summarised in Mant et al (2004)).
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The relative standard deviation for 11 replicate detections of 15 μM histidine was 2.7%.
Analyses of 36 replicate samples yielded 85 paired-replicate detections of analytes, and 6 unpaired replicate detections (a detection in only one of the paired samples).
In recent years several authors have used contact plates, Replicate Organism Detection and Counting (RODAC™) and contact slides (DIPSLIDE™), to detect and enumerate bacteria on environmental surfaces [ 8, 30].
GPNN was able to replicate the detection of a gene-environment interaction that had previously been detected using an exhaustive method, Multifactor Dimensionality Reduction (MDR) [ 59].
In parallel, we have developed a novel strategy to analyse the data that uses both genotype and pedigree information of all AIL individuals to replicate the detection of and fine-map QTL affecting juvenile body weight.
In order to maximize the power to replicate QTL detection and fine-mapping using an AIL produced from outbred founders, we propose that the genotypes and phenotypes of all the individuals in the pedigree and not just of those from later generations should be collected and analysed.
Laboratory A showed high variability between replicates using detection of the 3-day MN titers by CPE (91.7%), as the data from two replicate assays were comparable whereas the data from the third assay were inconsistent (data not shown).
In a NOEC analysis involving only few replicates, the detection of a small effect cannot be expected due to the small statistical power of a statistical test on binary effects with few replicates [14].
The agreement of technical and biological replicates on detection of exons was calculated using both simple agreement and Kappa statistics.
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