Sentence examples for replicate cycle from inspiring English sources

Each replicate cycle threshold (Ct) was normalized to the average Ct of the two endogenous controls on a per sample basis.

Each replicate cycle threshold (Ct) was normalised to the average Ct of five endogenous controls (B2M, HPRT1, RPL13A, GAPDH and ACTB) on a per plate basis.

For analysis of gene expression, the replicate cycle threshold (CT) values for each transcript were compiled and normalized to the geometric mean of the reference genes phosphoglycerate kinase 1 (Pglyceraldehyde, glyceraldehyde 3-phosphate dehydrogenase (Gapdh NM_008084), and hypoxanthine-phosphoribosyl transferase (Hprt NM_013556).

This demonstrates that the BoHV-4 replicating cycle is dependent on gB.

This observation suggests that the increase in SPREAD frequency requires the induction and/or synchronization of cells in the replicating cycle upon the second TPA treatment.

The SmartCycler II system is a PCR system by which amplification and detection are accomplished concurrently with TaqMan technology (Applied Biosystems, Foster City, Calif) using fluorescent probes to detect amplification after each replicating cycle.

Qiagen HCV quantitative kit was used to perform HCV RNA quantification with 10 ul of the extracted RNA on Roche Real Time PCR using fluorescent probes to detect amplification after each replicating cycle.

Few authors have tested preferences in less developed environments and/or societies with low exposure to Western culture, but among those who have, attempts to replicate menstrual cycle effects and relationship-context effects have been inconsistent (Yu et al. 2007; Penton-Voak and Scott 2010; also see Scott et al. 2008).

In the present study we have genetically engineered a fusion molecule encoding two chlamydial proteins; CT521 that resides in the cytosol of reticulate bodies during the replicating cycle (data not shown) and CT443 (omcB) located in the outer cell wall [41], [42].

They are obligate intracellular parasites that have two distinct replicating life cycle forms in the mammalian host.

We recently reported the full genome sequence of G. pallida [ 27] including a fully replicated life cycle transcriptome analysis and a preliminary description of the effectors likely to be present in this species.