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Due to the small size of the samples compared to the beam size, only a single replicate could be performed on each fragment, and the contribution of the embedding resin cannot be excluded.
The problem is that a failure to replicate could be attributed to many factors, only one of which is a poorly fitting model in the original analysis.
However, it should be noted that while the triplicate data of the SRSF screen means that errors present in a single offending replicate could be removed, the removal of edge effects in the HFA data leaves only one (or sometimes zero) remaining data point(s).
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Spearman rank correlations were determined using PROC CORR SAS Institutee 2003) for traits with a significant replication effect to determine whether the replicates could be combined.
Our results suggest that protocols based on spatially auto-correlated sign survey replicates could be used to monitor carnivore populations at large, and possibly even country-wide spatial scales.
If the data were consistent, then the results from the cytokine trial replicates could be compared with each other.
The interaction describing the replicates could be split similarly, with the remainder added to the error term of the analysis.
The results were based on 16 replicated simulations, which was computationally advantageous, since the 16 replicates could be run in parallel.
In this way the fidelity of the microarray technical replicates could be assessed (arrayMagic's R script, experiment description file and diagnostic plot are available on request).
The reason why the reference miRNAs did not decrease variability for technical replicates could be that the variability in the raw data was very low.
As the package does not allow for missing data, only transcripts represented by all 6 replicates could be tested by this method.
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