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In the second experiment, birds were assigned to three dietary treatments of five replicates with five birds per replicate (chamber).
Replicate chamber measurements were averaged by plot for each year of analysis.
A culture of planktonic cells with an average length of 1.6 μm underwent a morphological shift during the first 12 h of biofilm formation in replicate chamber slide wells.
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Mid-exponential phase (OD 600 nm = 0.4 to 0.5) replicate planktonic cultures of strain H1206 pJAM1020) were imaged, diluted (to OD 600 nm = 0.1) in fresh medium (Hv-YPC +0.5% glucose), and 500 μl aliquots were transferred to replicate chamber-slide wells.
Ten replicate chambers were used per test group.
From the treatment tanks, water flowed into the eight replicate chambers for each of the four treatment levels at 57.5 mL/min.
The effect of brood mass weight was added as a covariate, the replicate mating chamber was added as a random effect, and female identity (nested within the replicate mating chamber) was also added as a random effect in all models.
Temperature was maintained at 21°C and lighting was maintained at ∼258 and 280 minromoleach2/s−1 in each replicate growth chamber, respectively, for 16 hrs each day.
In this third model, female identity was nested within the identity of the replicate mating chamber.
Moreover, here, the random effect of the replicate mating chamber was important, so all models had this random effect in addition to the random effect of female identity (nested within the identity of the replicate mating chamber).
The random effects in the full model were 0.069 standard deviation for the replicate mating chamber and 0.115 standard deviation for female identity.
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