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In the following, the results of column A are shown, whereas the results of the replicate batch process (column B) are presented as additional files.
The application of bioreactor technology allowed highly reproducible culture conditions and physiological synchronization of replicate batch cultures.
Transcription profiles for the wild-type strain (HacAWT) and the HacACA strain were obtained using Affymetrix GeneChip analysis of three replicate batch cultures of each strain.
Three replicate batch fermentations with five experiments each were conducted under the optimal, low (run 15), high (run 17), and central conditions (runs 1, 3, 7, 9, 10, 13) (Table 4) to confirm the validity of the model obtained.
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A 23 full factorial design was built to different amoxicillin formulations, each one containing three replicate batches, and eight responses (physicochemical and biopharmaceutical parameters) were obtained.
Three replicate batches of 500 g dry barley were used for each strain as described above.
We took into account the possible natural variability between larval batches by collecting larvae from the adult barnacle cultures (consisting of several hundred individuals each) on four different occasions during two weeks in September-October 2011, representing four replicate batches of larvae (presumably with different parents, and thus different genotypes).
The biological aerobic degradation of 7 phenolic hydrocarbons (phenol, o-cresol, o-nitrophenol, p-nitrophenol, 2,6-dichlorophenol, 2,4-dichlorophenol, 4,6-o-dichlorocresol) and 1 aromatic hydrocarbon (nitrobenzene) was studied for 149 days in replicate laboratory batch microcosms with sediment and groundwater from 8 localities representing a 15 m × 30 m section of an aerobic aquifer.
The biological aerobic degradation of 7 aromatic hydrocarbons (benzene, toluene, o-xylene, p-dichlorobenzene, o-dichlorobenzene, naphthalene and biphenyl) was studied for 149 days in replicate laboratory batch experiments with groundwater and sediment from 8 localities representing a 15 m × 30 m section of an aerobic aquifer.
Data points represent the mean and standard error of five biological replicate seed batches.
Approximately, 25 50 freshly harvested seeds from each plant were sown onto 0.9% water agar plates, and at least five plants per treatment were used to generate biological replicate seed batches.
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