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Data were expressed as the mean spot-forming cells (SFC) of 4 replicate assay cultures.
Each replicate assay used 11.25 ng total cDNA in a 20 µl final volume.
50 ng total cDNA (as total input RNA) in a 10 µl final volume was used for each replicate assay.
We tested overall preference of caterpillars (mass test diet eaten - mass control diet eaten) for native versus exotic plant extracts with mass of the assay caterpillar as a covariate to control for the total amount of feeding in each replicate assay.
A list of top 100 resistant and sensitive candidates was assembled from all plates and was compared to a replicate assay.
For these reasons, estimated sensitivity of the three replicate assay is likely to be smaller than true sensitivity; the effect on specificity is less clear but probably small.
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Three replicate assays for each sample were independently processed.
At least three replicate assays were performed for each toxin and strain evaluated.
Three replicate assays were performed and the activity relative to p210 wt was calculated.
Expression data from replicate assays were averaged with the GEPAS microarray preprocessing software and logarithm (base 2 -transformed.
Seven replicate assays were performed for each of the 4 treatments: male, female, infected and uninfected and the threshold cycle value (Ct) was averaged across these replicates.
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