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Three replica within a distance of 1 m were withdrawn and kept in open plastic bags in the dark at 4°C for 40 hours.
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Replicas within a chosen region.
The centroid has been computed as the median value among the replicas within the same set of data.
Under these conditions these extremely divergent sequences were mostly (80% of the cases in 100 replicas) within the out-groups, and only rarely (< 5%) in the position of microsporidia in our favored scenario.
Data was analyzed for expression relative to GAPDH using the comparative Ct method with data resulting from the average of three replica wells within each experiment.
Values of allelic ratios from experimental replicas fell within the same range, showing the robustness of the quantification method (Figure 3D).
The intra-assay reproducibility was assessed by comparing the results between the two replicas printed within the same chip for each clone and gave an R2 = 0.9311.
Local people were encouraged to open shops and restaurants, but the company then built a replica of the town within the pier complex.
In another part of the palace, he not only had a copy of one of the most renowned Greek statues, the fourth-century BC nude Aphrodite from the town of Cnidus, by Praxiteles (reputed to be the first Greek female nude ever), but he displayed it within a replica of the very temple in which she was kept in Cnidus.
Field validation trials further assessed the efficacy of heat treatment within a replica sea chest environment.
Omori says he'll be recreating some of these scenes, whatever they may be, within his replica, as the season unfolds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com