Exact(6)
(b) Replica sample of 1% NaCl solution without MNBs shown as a control.
(c) The replica sample of pure water without aeration was shown as a control.
The details of the replica sample preparation were mentioned elsewhere [20], so we explain them just briefly here.
We prepared the replica sample for this solution just after its delivery, when it took more than one week after the MNB formation.
In contrast, when the replica sample was prepared on area (c) including the MNB surrounded by purified water (PW), the observed TEM image was (c') the MNB adsorbing fine particles on its surface in smooth ice crystallites (related to Figure 5).
Detailed results for the original Spanish GWAS dataset, Spanish replica sample, and the combined Spanish dataset are described in Additional file 5. We fitted a multivariate logistic regression model for the combined Spanish sample in which we adjusted for age, sex and APOE.
Similar(54)
When this solution is quenched and the replica samples are prepared on area (b), no MNBs with homogeneously dispersed impurities were observed.
Several parts of the replica samples prepared from the wastewater had a rough surface including many fine particles (less than 10-7 m in diameter) as depicted in Figure 4a, b.
Based on these TEM images of replica samples from the wastewater (Figures 4 and 5), the impurity adsorption of MNBs in the wastewater can be described as follows (Figure 6).
TEM images indicated that most of the observed areas on the replica samples for the pure water including O2 MNBs were smooth, and that a small number of objects were observed.
The size distribution of MNBs obtained from TEM images of replica samples prepared just after aeration (solid circles with arbitrary unit, n = 114) is similar to that measured by a dynamic light scattering method (open diamonds with error bars and a smoothed line), which was reproduced from Ushikubo et al. [19].
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