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At the end of the cooling process the main conformations present in a given replica population are identified by performing cluster analysis.
For instance, an association study in East Asians could have failed to replicate a previous association found in Europeans if, with similar sample size, the tested genes harbored markers with lower allele frequencies in the replica population.
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Further, cheat frequencies showed a consistent increase over time in mutator populations, rising to dominance in all six replica populations; concomitantly, there was little variance in cheat dynamics between replica populations.
Probably 80 percent of the population wears replica Hawks home sweaters, transforming the balcony into a circular sea of red.
With all this in mind, the first objective of the paper is to analyse whether all the information given by the CSWL on the benefit recipients makes up the best replica of the study population that researchers can have.
These were created by nonreciprocally crossing two near-isogenic lines [derived from a replica of the LHM population fixed for an autosomal recessive mutation that encodes brown eyes (Friberg and Dowling 2008)].
PAO6049 is a methionine auxotroph and so the two strains may be distinguished in mixed populations by replica plating on CAA and M9 minimal agar.
When considering only the three residues that form the specificity determining segment: Glu pTyr+1 -Glu pTyr+2 -Ile pTyr+3), the average RMSD goes from 5±2 Å at 3000K (Figure 2A and 3A) to 3±2 Å after cooling at a rate of 50 ps/step, to 2±1 Å at 500ps/step, with 72% of the population (36 replicas out of 50) in a cluster with an average CA-RMSD of 1±0.5 Å (Figure 2B C and 3B).
Two sets of experiments were performed: i) two replica runs per strain and population size were done in parallel for strains N2, CB4856, AB1, JU258, MY1, MY15, MY18 and RC301 using population sizes of 75 and 150; and ii) five replica runs per strain and population size were done in parallel for N2 and CB4856 using population sizes of 40, 70, 100 and 150.
In both populations, a skin replica was taken laterally to the left eye in the crows feet region of the face and its topography analyzed.
In both sexual and asexual populations, mutator frequencies were assessed by replica plating 100 to 300 randomly sampled colonies, derived from each population at each assayed time point, from YPD plates to YPD plates containing 200 μg/ml of G418.
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