Exact(10)
We found a clear threshold behavior in the initialization of pesticide transport that was different between the replica experiments.
These analyses underscore the need for replica experiments to reliably interpret large-scale expression data sets, even with simple microarray experiments.
To further minimize experimental error and limit false positive results, three biological replica experiments were prepared and independently analyzed.
The validation of both CD81 and CD44 by independent methods confirms our previous conclusion that three biological replica experiments give a high level of confidence for the specificity of the results [14].
Each condition was evaluated from at least two biological replica experiments using pbMEC from different cows.
However, the following differences of the pathogen-specific induction of the candidate genes were concordantly found in replica experiments.
Similar(50)
A replica experiment was designed to shed light of this issue and provided new information on the material constituting the bead not measurable in a non-destructive way.
The pool of RNA samples from G93A-SOD1 spinal cords harvested at 30 minutes from compression injury was also used for a technical replica experiment.
The r2 value of the technical replica experiment performed probing two separate bead-arrays with the same RNA sample was 0.9876.
A technical replica experiment is also reported, based on the comparison of the gene expression profiles obtained from the same spinal cord sample (extracted at 30 minutes from compression injury) used to hybridize two separate Bead arrays (R: 0.9872*).
500 ng from each of the 14 RNA pools were hybridised simultaneously to 16 arrays (including 2 arrays for the technical replica experiment) contained in 2 RatRef-12 Expression BeadChips, as per Illumina protocol (Illumina, San Diego, USA). the Bead-chip output files were analyzed using the BeadStudio-3 San Diego(IllUSAnas San Diego, USA) as previously reported [ 6].
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