Sentence examples for replenishment of medium from inspiring English sources

Then, scaffolds were incubated in DMEM for 21 days with regular replenishment of medium every alternate day.

First, most static culture methods do not allow for the constant replenishment of medium and important nutrients.

The plates were incubated for 14 days in a 5% CO2 incubator at 37 °C, with replenishment of medium every 3 days.

The Rho cell line, obtained by culturing 143B.TK− in routine growth medium containing 50 ng/ml ethidium bromide with regular replenishment of medium for about 2 months, was maintained in DMEM supplemented with 10% FBS and 0.2 mM uridine (Sigma).

The replenishment of medium and ZD1839, 48 h through the 5 day period was performed to ensure consistent drug exposure for the duration of the assay, since our preliminary experiments with ZD1839 alone were based on a 72 h incubation and previous experiments with ZD1839 in vitro have used drug replenishment (Ciardiello et al, 2000b).

Media change was performed twice per week, with complete replenishment of chondrogenic medium defined above.

Interestingly, CHO cells expressing Bcl-xLΔ were also able to recover and return to rapid growth rates much faster than either the wild-type CHO-bcl-xL or CHO following the replenishment of fresh complete medium containing 10% FBS.

Of note, when the CFE assay is applied, cells are exposed for 24 h to nanoparticles, followed by wash-out of the particles and replenishment of cell culture medium, and the number of cell colonies is counted after 7 days, as an indicator of cell survival/cell proliferation.

Removal of mono-chlorobenzene (m-CB) vapors from air streams in a biotrickling filter unit was studied under various operating conditions concerning inlet m-CB concentration (Cgi), air residence time, liquid recirculation rate (Q), pH, and frequency of medium replenishment.

The next day the medium was changed and the As2O3 was added (concentrations of 0.5 2.5 μM), with no further changes of medium or replenishment.

Replenishment of cell cultures with full medium increased cell number in both cell lines, although it was more marked in siHIPK2 cells.