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The final models were then used in molecular replacement to solve the crystallographic phase problem.
Subsequently, this model was used in molecular replacement to solve the structure of Pat-C at 3.1 Å resolution, with four molecules per asymmetric unit in a different packing environment (Rwork=24.8%, Rfree=28.1%, crystal form II; Supplementary Table S2 and Supplementary Figure S4A).
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Ultimately, molecular replacement was used to solve the structure by sorting through a large number of solutions in space group P1 using CNS.
The crystals diffracted to a resolution of 1.76 Å (Table 2), and molecular replacement was used to solve the ChsH1-ChsH2N/3-OPC-CoA strusinge using the apoprotein structure as the template.
If the sensor replacement was unable to solve the problem, try to focus your attention on the thermostat.
Neither the available structures of PNKP FHA domain (PDB: 1UJX, 2BRF) nor that of aprataxin (PDB 3KT9) proved useful for phasing of these X-ray data by molecular replacement but we were able to solve the structure using the SAD method and crystals of selenomethionine-substituted protein and refine against data extending to 1.4 Å resolution (Table 1).
Contribution: This article presents the first efficient algorithm to solve the contact replacement problem for entire proteins.
Still, astronomers lamented that the spacecraft's loss would leave them with as many mysteries as discoveries, and they are not likely to solve the mysteries without replacement spacecraft.
It becomes inevitable that companies need to invest their time and money on new/replacement employees who need to solve the same problem again and again.
The structure of M. tuberculosis TreS was solved by molecular replacement to a resolution of 2.6 Å.
In each case, the protein crystallized in the space group, P43212, and the structures were solved by molecular replacement to resolutions ranging from 1.6 to 2.2 Å, with refinement statistics given in Table 1.
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