Sentence examples for replacement sequence from inspiring English sources

Sequence-replacement mutants were generated either by ligation of a double-stranded oligonucleotide sequence into a linearized construct lacking the sequence to be replaced or by performing PCR with primers that contained sequence corresponding to the replacement sequence at their 5′-end (see Supplementary Figure S1 at http://www.biochemj.org/bj/456/bj4560139add.htm).htm

Replacement of the HT motif with an alanine-rich replacement sequence blocked release of GFP into the erythrocyte cytoplasm (Fig. 3B).

Yeast cells that are resistant to 5-FOA are screened by PCR for the precise insertion of the replacement sequence.

Multilocus sequence typing (MLST) analysis of pediatric PnEmp cases in Utah (USA) suggested that replacement sequence types (STs) contributed to the increase in incidence of pediatric PnEmp post-PCV7 [ 31].

Sequencing across the insertion site was deemed unnecessary as the replacement sequence was introduced into MW005 as a restriction fragment [ 10] rather than a PCR product as used previously [ 5].

These plasmids were designed as pairs the first member of which provides the template for PCR-amplification of the RT-cassette, used in the first, positive selection recombineering step, while the second provides the desired replacement sequence, excised as a restriction fragment, for use in the subsequent counter selection step.

Trans-splicing strategies for mRNA repair involve engineered transcripts designed to anneal target mRNAs in order to interfere with their natural splicing, giving rise to mRNA chimeras where endogenous mutated exons have been replaced by exogenous replacement sequences.

It is now accepted that most of these purported cases of succession in the fossil record were really replacement sequences consisting of the remains of different local or regional ecosystems, paced by environmental fluctuations often caused by large-scale climatic and geologic processes (Miller 1986).

For obsolete sequences, we downloaded the replacement sequences, but often the whole-genome sequence is used as a replacement.

We then built new single-gene trees to confirm that the appropriate replacement sequences had been found, by confirming the monophyly of the eukaryotic clade.

The struthiolariids exhibit a species-replacement sequence in which they become smaller and thinner-shelled upsection, but apparently not in association with the cooling event [17].