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What about the replacement of copies with real material, like your Hoffman chair?
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We confirmed the complete replacement of native copies of the chromosome by mutated copies by amplification by PCR (Supplementary Fig. S1) with the pairs of primers listed in Supplementary Table S2.
Horizontal transfer with replacement of old copies or reactivation may have occurred.
The two genomes, thus reduced by the replacement of single-copy genes by a lesser number of dummy genes, is then examined for excess adjacencies.
In the latter organisms, however, competition between centromeres is expected to lead eventually to the replacement of low-copy centromere sequences with repeat-rich centromeres of well-positioned cenH3 nucleosomes.
Replacement of both allelic copies of TcNMT (T. cruzi NMT) was only possible in the presence of a constitutively expressed ectopic copy of the gene, indicating that this gene is essential for survival of T. cruzi epimastigotes.
This means that the occurrence of diseases associated with the replacement of active gene copies with defective sequences have to be regarded as a trade-off in the gene correction model (Bischof et al., 2006).
We examined the extent of replacement of wild-type copies of individual genes after each mutagenesis by PCR, using DNA isolated from wild-type and mutant cells as templates and appropriate forward and reverse primers (Fig. 1 and Supplementary Table S2).
C57BL6 CX3CR1+/GFP mice that harbor a targeted replacement of a single copy of the CX3CR1 gene by a GFP-reporter gene were kindly provided by Prof. Jung Jungg et al. 2000).
Tempers cooled a little a day later when a group of settler rabbis delivered replacement copies of the holy book, in an unusual gesture of reconciliation.In this section Unholy rows Deep waters, slowly drying up Trial trails Passing the tin ReprintsElsewhere in Jerusalem and the West Bank, the mood is at its bleakest in places which more than one faith deems sacred.
However such transfers could have occurred only recently by homologous and non-homologous replacement of viral-type ancestral copies in NCLDV.
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