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Correct deletion of the target genes was detected by diagnostic PCR using whole yeast cells from isolated colonies and a set of oligonucleotides designed to bind outside or inside of the replaced target sequence and within the marker module.
The homologous recombination strategy replaced target genes by selection marker genes.
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Non-native speakers are likely to replace target language phonemes by phonemes from their mother tongue [3, 5].
Genome-wide targeted gene deletion, a systematic method to study gene function by replacing target genes with deletion cassettes, using serial-PCR or block-PCR requires elaborate skill.
In a horrible year like 2008, the banks replacing target bonuses with fully competitive salaries would have had far higher compensation expenses.
Coordinate system manager of the viewer replaces target area of the target area manager and sensor of the node manager with MSNS screen coordinate based on the map coordinate that is set in the map layer manager.
While many research projects tend to emphasize the potential of that process in replacing target bits of genetic material, for Mammoth Biosciences, the search function is the real game changer.
Experiments demonstrate that on LUT-based FPGAs, the SPFD-based is more powerful than the ATPG-based rewiring scheme in finding useful alternative wires to replace target wires for delay improvement, under comparable CPU time.
The mutant models (H251A, H284A and H297A) were built from the native models by replacing target residues with the desired amino acid using Discovery Studio 2.5 (Accelrys Inc).
Such trials were otherwise identical to those of the main experiment, incorporating the four change conditions (replacing target noise with a blank region where appropriate).
The nontemplate controls, with water replacing target molecules, displayed that also the amount of nonspecific PCR product increased with assay number.
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