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Briefly, animals were placed on a small island (3.5 cm diameter) in a pool of water for the entire 72 h period and replaced onto the island whenever they fell into the water.
The glial medium was replaced onto a predominantly astrocyte monolayer.
T-cell media were replaced onto the PG13 monolayer with more virus collected and filtered after an overnight incubation for second and subsequent transductions.
After an additional 12 h, the cells were replaced onto a silicone chamber coated with fibronectin at a density of 3 × 10 cells/well, and cyclic tensile strain was applied.
Cultures were harvested after 24 hrs by gentle filtration, and replaced onto fresh MA medium with either L-arabinose, erythritol or xylitol as an inducer (10 mM), and incubated for further 12 hrs.
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Then rinse in tap water and replace onto the cooler.
The washed off larvae were individually replaced back onto the first leaf-blade of a fresh seedling of the susceptible Newton.
The problem can be solved effectively by using the modified subgradient extragradient method proposed in this paper, since two projections onto the original domain are all replaced with projections onto some half-spaces, which is very easily calculated.
The main modification is to use the subgradient of a convex function to obtain a hyperplane, and the second projection onto the intersection of the feasible set and a halfspace is replaced by projection onto the intersection of two halfspaces.
In order to overcome this weakness, Censor et al. developed the subgradient extragradient method in Euclidean space [4], in which the second projection in (1.4) onto C was replaced with a projection onto a specific constructible half-space, actually which is one of the subgradient half-spaces.
It has been used in hundreds of studies of airborne pollen and plant- or fungal-spores, particularly in modified forms such as the Burkard seven-day spore trap, which replaces collection onto a glass slide by collection of particles onto adhesive tape on a circular drum that rotates over a period of seven days.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com