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To avoid repetitive sequencing, the dynamic exclusion of sequenced peptides was set at 10 s.
Sequencing-by-synthesis technologies generate data by repetitive sequencing, or oversampling, of a given DNA segment and can be adapted to sequence one particular DNA region at great depth [1] [3].
A portion of the cDNA library was subjected to normalization using a duplex-specific nuclease (DSN) in order to avoid repetitive sequencing of highly expressed genes [ 36, 37].
In order to avoid repetitive sequencing of identical clones, i.e. to reduce the library redundancy, normalization of the libraries was done using the following strategy.
Before sequencing, RFLP, using EcoRI+ SacI and HindIII, as in Selosse et al. [ 5] was investigated to avoid repetitive sequencing of the same ITS.
In future experiments, to avoid the repetitive sequencing of this abundant mRNA, we plan to introduce, during cDNA insert amplification, interference primers specifically designed for the 16S rRNA [ 17].
Similar(52)
Two additional variable regions contain repetitive sequences with varying number of repetitions.
Their themes come in repetitive sequences.
A repetitive sequence such as AAAAA contains little entropy.
The smaller the pieces, the harder it is to solve the puzzle, particularly in places where there are repetitive sequences.
Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown.
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