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He mentioned that advances in the development of non-invasive and repetitive quantification of liver fibrosis can be attributed to progress in serum fibrosis markers and ultrasound-based transient elastography rather than the classical biopsy-based semi-quantitative scoring system of liver fibrosis.
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It should be mentioned that methylation quantification of other repetitive elements, such as Alu repeats, is sometimes performed to assess global DNA methylation [ 18], which was not measured in this study.
A promising approach was developed where data from amplification of repetitive sequences were used in relative quantification of species in binary mixtures.
Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown.
DNA quantification utilised PCR of the repetitive Line1 79 bp fragment, as described by Diehl et al (2008).
Some molecular analyses have consistently shown a difference between indica and japonica in the quantification of genomic DNA and repetitive sequence [ 40].
We also quantified the gene expression when additionally accounting for SV, alternative splicing and repetitive sequences that can all bias gene expression quantification.
Quantification of the fluorescence before and after repetitive photobleaching was performed in 2 circular areas with a diameter 1 µm in the myofiber: 1] an area that coincides with the bleach spot and 2] an area in the domain adjacent to the domain in which repetitive photobleaching was performed.
Two different targets were chosen for quantification: a housekeeping gene, β-actin, and Alu repetitive elements.
The figure legend was not repetitive, it merely identified the raw data panel and the quantification in the histogram.
The repetitive character of human Alu-sequence RNA allows a highly sensitive detection and quantification of human disseminated viable tumour cells in xenograft mouse models using qRT PCR.
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