In experiments where the cells were stimulated with repetitive addition of 10 μM HNE, however, PGE2 release and COX-2 protein expression were enhanced after 4 hours of incubation, with maximum values obtained at 24 hours.
For COX-2 and mPGES-1 studies, human osteoarthritic chondrocytes were stimulated at different incubation times (up to 24 hours) with a single or repetitive addition of 10 μM HNE to the cultures at 2-hour intervals, up to 14 hours.
In each of the microcosms, the DGGE separation patterns varied over time, which suggests that the structures of the 3-CB degrading communities were not static, even after degrading repetitive additions of 3-CB degrading
While flying around the city and running faster than cars is fun, missions are mostly rehashed from previous games, which can make the experience feel repetitive, and with the addition of super powers, it will provide little challenge for fans of the series.
The fourth generation, amine terminated hyperbranched PAMAM was grafted on MWCNTs, which was achieved through repetitive reactions of Michael addition of methylmethacrylate to the surface amino groups and amidation of terminal ester groups with ethylenediamine.
Telomerase is a multimeric ribonucleoprotein enzyme that catalyzes the addition of repetitive DNA sequence to telomeres, specialized structures at the ends of chromosomes.
Despite MEUX having a much smaller gene content than HSAX and being regarded as the ancestral therian X [ 21], its DNA content is comparable with HSAX, due to the addition of repetitive sequences of this NOR-bearing chromosome.
The RNA pellet was washed twice with 80% ethanol, dried, and complete dissolution was obtained by addition of water, repetitive pipetting, and incubation for 15 min at 65°C.
In line with these findings, the LPI-induced inward current (repetitive stimulation) was reduced upon addition of 2 mM Ni2+ into the bath by 50±6%6%.
Our results show that dRNA-seq may be used to capture some of the modifications at the transcript 5′ end, allowing a more systematical characterization of the metabolic factors that regulate gene expression by acting on the extent of repetitive nucleotide addition or on the choice of the initiating nucleotide.
In this study, the addition of PEEP prevented repetitive recruitment and derecruitment in both the nondependent and dependent lung regions.
