Arakaki, A. et al. TCR-β repertoire analysis of antigen-specific single T cells using a high-density microcavity array.
Our results suggest that repertoire analysis of antigen-specific TCRs can be an important readout to assess whether a vaccination was able to generate memory cells in clonal sizes that are necessary for immune protection.
(a-f) High-throughput sequencing and heavy-chain repertoire analysis of IgG1 and IgE in sorted B220+CD95+GL7+ GC B cells and FSChiCD95hiCD138+ PCs from skin draining LNs of wild-type and Tcrd-/ mice 7 days after the last of two topical exposures to DMBA (n=6/group).
Ferdman, J, Palladino, G, Liao, H-X, Moody, MA, Kepler, TB, Del Giudice, G, Dormitzer, PR, Harrison, SC, Settembre, EC, and Suphaphiphat, P. "Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine". Vaccine 36, no.
Repertoire analysis of immature, transitional and naïve B cells of patients with SLE and non-autoimmune individuals has confirmed the presence of multiple tolerance checkpoints [14], [23], [24], [25].
We showed that despite the strong dominance of DP lymphocytes expressing one particular Vβ chain (2, 11 or 13.2) in two out of four melanoma invaded lymph nodes (M134 and the M314), the repertoire analysis of these DP T cells populations was relatively diverse and did not reveal any recurrence of a particular Vβ usage.
Functional T-cell assays looking at cytokine production or proliferation in response to antigen or TCR repertoire analysis are of interest to better understand pathogenesis but have not been clearly linked to clinical prognosis or diagnosis.
In our previous work, we described the clinical characteristics of patient OE as well as TCR repertoire analysis and sequencing of the TCR Vβ domains of patient OE [15].
In this study we used the same protocols for TCR repertoire analysis and sequencing of the TCR Vβ domains of patient VB.
Because of the critical importance of data analysis for high parameter single cell analysis, we discuss essential factors in analysis of these data, including quality control, quantification, examples of methods for high dimensional analysis, immune repertoire analysis, and preparation of analysis pipelines.
Recently, a deep sequencing-based TCR repertoire analysis revealed a panorama of various adaptive immune responses18.
