Sentence examples for repeats were generated by from inspiring English sources

CAG and CUG repeats were generated by ligating together shorter repeat oligonucleotides and expanded using polymerase chain reaction (PCR -based techniques as described PCR -based (8).

An alternative possibility is that the repeats were generated by a single break in the centromere-distal location, followed by a BIR event in which the centromere-proximal site was invaded.

Although we do not need to invoke this model for most of the repeats, it is possible that the Type 1 repeats were generated by this mechanism (termed "synthesis-dependent microhomology-mediated end joining").

Although we favor the model in which the CUP1 repeats are generated by "classic" NHEJ, this model does not explain the A/T base pair that is present at the junction of the Type 1 repeats.

In several neurodegenerative disorders, such as spinocerebellar ataxias and Huntington's disease, long pure glutamine repeats are generated by the instability of CAG codons [ 25- 27], and cause the abnormal proteins to form intracellular inclusions in specific neuron types.

The more common 4-repeat allele has been identified as the conserved ancestral allele [6], while the 7-repeat was generated by a rare mutational event and the 2-repeat is the product of a single recombination event between the 4-repeat and 7-repeat alleles [5].

rAAV (rAAV1/2) mosaic vectors containing a 1 : 1 ratio of AAV1 and AAV2 capsid proteins with AAV2 inverted terminal repeats (ITRs) were generated by crosspackaging as previously described.

DNA samples containing various CAG repeat lengths were generated by PCR from tail snips of either wild type or R6/2 mice [16], which carry the first exon of the human HD gene with a pathologically expanded repeat.

Multiple alignments of repeat families were generated by RIPCAL [ 16] using ClustalW [ 71, 72].

No AHA1 or AHA2 protein interactions were identified in the yeast two-hybrid interactome database, and only two high-confidence candidates, both annotated as leucine-rich repeat kinases, were generated by the split ubiquitin approach.

The ASB9 ANK repeat deletion mutants were generated by PCR using the following primers in combination with ASB9 3' primer: ASB9ΔANK1 5'-primer, 5'-CTCGAGATGGATCATGTTTCCCCACTCC-3'; ASB9ΔANK1-2 5'-primer, 5'-CTCGAGATGGACTGGCACACTCCACTG-3'; ASB9ΔANK1-3 5'-primer, 5'-CTCGAGATGCTGGCATCCCCCATCCAT-3'; and ASB9ΔANK1-5 5'-primer 5'-CTCGAGATGCAGGATTCCCCACTTCATG-3'.