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Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo.
Telomerase synthesizes new telomere repeats onto chromosome ends to offset in part this telomere shortening [11], [12].
Telomerase synthesises (TTAGGG n DNA repeats onto chromosome ends.
Telomerase is a reverse transcriptase that contains an RNA template used to synthesize telomeric repeats onto chromosomal ends.
This enzyme, a reverse transcriptase, adds back telomere repeats onto the ends of chromosomes and thus counteracts telomere attrition (Greider, 1990).
A well-established function of telomerase is to synthesize telomeric repeats onto chromosomal ends and thus be responsible for the telomere maintenance [ 2].
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The verification process is iteratively repeated onto each smaller hyper-cube until the stopping criterion is satisfied.
Briefly, the telomerase in the cell extract from 1×105 cells of SCAP, DPSCs, or BMSSCs added telomeric repeats (TTAGGG) onto the 3' end of the substrate oligonucleotide and iQ SYBR Green Supermix BioRad Laboratoriess, Hercules, CA), and amplified with an iCycler iQ real-time PCR machine (BioRad Laboratories).
In tree peony, the abundance of tri-nucleotide repeats mapping onto CDS regions was consistent with results found for the six species studied by Sonah et al. [ 19].
Telomerase activity, in terms of the addition of telomeric repeats (GGTTAG) onto the 3′ end of a substrate oligonucleotide (TS) during an initial reaction, was then calculated by real-time qRT-PCR.
In some cases such as in [30], an imprint replica of the master mold is first obtained using nanoimprint lithography (step-and-repeat technique) onto a resist-coated wafer, where a nickel layer is then deposited onto the imprint and peeled off to be used as the flexible mold in the imprint process published in [42].
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