On record Mr. Lupu again and again turns these repeats into intimate dramas.
And I hear the guy ask my mom, "So how much pot does she smoke?" And mom just repeats into the phone, unaware of what she is actually saying.
In addition to natural amplicons, methodologies have been developed to engineer direct repeats into genomes.
Two mechanisms are proposed to cause FXTAS: RNA gain-of-function, where CGG RNA sequesters specific proteins, and translation of CGG repeats into a polyglycine-containing protein, FMRpolyG.
Overall, these results suggest that translation of expanded CGG repeats into FMRpolyG alters nuclear lamina architecture and drives pathogenesis in FXTAS.
Marcus, 55, and his business partner, Ira Bernstein, 50, are the Click and Clack of Debmar-Mercury, a television distribution company whose success was formed by turning rejected South Park repeats into a thriving business.
Here, we show that incorporation of tandem nuclear localization signal (NLS) repeats into the ZFN protein backbone enhances cell permeability nearly 13-fold and that single administration of multi-NLS ZFN proteins leads to genome modification rates of up to 26% in CD4+ T cells and 17% in CD34+ hematopoietic stem/progenitor cells.
The identification of different repeats into the promoter regions studied may be suggestive of specific response regulator binding sites.
The purpose of MDA is to sort the highly variable cross-bridge forms within the repeats into self-similar groups.
To construct expression plasmids encoding the Rnq-polyQ fusion proteins, we first inserted htt exon 1 PCR fragments with 19, 54 and 91 CAG repeats into the NotI and EcoRI sites of pYex2T/L, thereby creating plasmid intermediates.
siRNA mediated reduction of hnRNP H, which binds to the base of the CUG hairpin, has also been demonstrated to allow transport of RNAs encoding expanded CUG repeats into the cytoplasm [51].
