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For the industrial production of β lactams, the filamentous fungus Penicillium chrysogenum has been subjected to an intense classical strain improvement (CSI) program that consisted of repeating rounds of mutagenesis and selection.
By exploring larger sequence regions, adding and removing sequences, and repeating rounds of search and realignment, we found the TTTACA sequence to be the 3'-end of a 17 nucleotide motif, motif 1. Motif 1 is sometimes repeated, as seen in the region before Rru_A3095, the only PEP-CTERM gene in R. rubrum.
Using a systematic fashion of repeating rounds, where each subsequent set of statements is built on the responses to the preceding ones, consensus is sought through the feedback of information and iteration [ 2], and the process is terminated when consensus is reached.
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He arranged the facade around repeating round-arched bays of windows and filled in the spandrels under the windows with hypnotically repeating patterns of brick.
After repeated rounds of trade liberalization since World War II, industrial tariffs have fallen to an average of 4percentt worldwide.
Meanwhile, repeated rounds of gunfire rang out (one of which eventually led to the incarceration of Li'l Kim).
The two scientists will then play repeated rounds of "dice war," with a point awarded each round to the player rolling the higher number.
City officials were quick to say the police had not fired any live rounds, but riot police personnel did fire repeated rounds of tear gas.
Coming after repeated rounds of belt-tightening as the government struggles to pull the country out of its debt crisis, the measures have been deeply unpopular.
Another 20 minutes on, police turned on a high-pitch siren and fired repeated rounds of stun grenades into the crowd along with an even bigger gas bombardment.
The agreement was to have constituted a basis for a permanent truce between Israel and Hamas, which would prevent the repeated rounds of shooting.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com