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So, in theory, the reductases could be distinguishable by repeating batch cultivations and using growth as selection.
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Three modes of operation are considered: continuous, repeated batch, and repeated fed-batch operation.
Fig. 5 Repeated batch fermentation with S. roseosporus cells.
The system was operated in repeated batch modes, for four cycles.
The candidates for optimal mode of operation are shown to be either steady-state continuous, repeated batch or repeated fed-batch operation.
Moreover, the ME yields remained above 85% after 20 repeated batch cycles.
The frequency of repeated batch operation was controlled by the measured fermentation redox potential.
Moreover, Fe2O3@SnO2 nanocubes can maintain their catalytic efficiency during the repeated batch experiments.
The catalyst α-FeOOH maintained its high activity during repeated batch experiments.
Despite this, there have been few detailed studies involving the repeated batch cultivation of S. platensis.
The Novozym 435 was reusable and performed effectively in castor oil conversion as shown in the repeated batch experiments.
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