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In addition, repeated washing cycles considerably improved the results.
Without standards, professional cleaners say, it is easy to bungle a job that often requires gutting and repeated washing.
The exponential phase bacterial cells have been collected through centrifugation at 10000 rpm for 10 min and repeated washing.
Precipitates were obtained by centrifugation and repeated washing (five times with DI water and once with anhydrous ethanol).
Because percale has one horizontal yarn for every vertical yarn in the weave, it results in a more breathable sheet, which can stand up to repeated washing.
By aspirating the supernatant and repeated washing, we obtained rod-shaped myocyte population.
Human blood was obtained by venipuncture, and erythrocytes were immediately isolated by centrifugation and repeated washing in sterile HBSS.
After printing on a streptavidin-coated slide and allowing the print to dry, we observed no cell translocation throughout many repeated washing steps.
Ionically bound CS-A was removed from the Sepharose by repeated washing steps of alternating 0.1 M acetate buffer, pH 4.5, containing 0.5 M NaCl and coupling buffer.
Repeated washing steps with TBS containing 0.05% Tween 20 were inserted between all incubation steps.
Following repeated washing steps, 100 μl of conjugate were added into each well and incubated for 1 hour.
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