Sequence analysis of the transcribed genes suggested repeated transpositions at protracted intervals.
We did not find any region matching the criteria for definition of a genomic island, i.e. differing G+C content, presence of direct repeats, transposition elements or tRNA sequences [25] within the CS-505 and D9 genomes.
Comparative analysis of an NBS-LRR cluster in Phaseolus vulgaris and other Fabaceae indicates that subtelomeric positions are prone to transpositions of repeated DNA elements [ 45].
An engineered piggyBac transposon with minimal length 5′ and 3′ terminal repeats exhibited greater transposition activity in transfected cultured human cells than a well-characterized hyperactive SB system.
We began with Kc167 cells, which were used in the earlier work on P-element transposition in cells, and subsequently repeated the procedure with the S2 derivative Sg4.
It appeared also, that the mini-F transposition into positions of the unique regions is more efficient than into the repeated regions of the VZV genome.
We argue that repeated changes in the phenotypic optimum in a multidimensional fitness landscape may induce explosive bursts of transposition activity associated with faster adaptation.
We have shown that structure formation by a Friedreich ataxia patient-derived GAATTC repeat tract directs Tn7 transposition to the repeat in vitro.
Deletion of the terminal sequences including the sub-terminal repeats decreased the transposition activity of the 708-residue-long transposase, while the transposition activity of the short form of transposase was not affected.
DNA sequence analysis reveals that TIGD1 retains the conserved catalytic DDE/D core but lacks one of the terminal inverted repeats necessary for transposition (data not shown) [ 21].
