Exact(1)
ade12 mutants did not produce Ura+ colonies even after repeated transformations with pRS316 and YCpGal:RIb plasmid DNAs.
Similar(7)
The accumulation of misorientations was continuously in progress up to 500 repeated transformation cycles.
The knockout mutants for the hypothetical proteins Synpcc7942_1561 and Synpcc7942_1023 could not be obtained despite repeated transformation attempts, indicating that these genes may be essential for cell growth.
All combinations of fusion constructs scoring positive for an interaction were validated by repeating transformations and selection on dropout medium.
The volume fraction of nanoscale crystals is dependent on complex relationships of different factors chemical composition of metastable alloys, rate of cooling and following heating in the course of the direct γ α, and the reverse α γ transformations, the number of the repeated γ α γ transformations (level of the phase hardening) [1, 2, 4 6].
In addition, analyses were repeated using transformations (square root and log 10, adjusting for zero scores) of the skewed outcomes (medication use, healthcare utilization, and life satisfaction) to normalize their distributions.
As previous investigators used square-root transformation of CD4+ T-cell count to stabilize variance, the final model was repeated with transformation to ensure inferences were unaltered [4], [13].
During construction of the hrk1Δ+HRK1 mutant, repeated biolistic transformation may affect its normal virulence (Fig. 7C).
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