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Alternatively, these results could be explained by repeated template switching, as has been observed in Saccharomyces cerevisiae (Tsaponina and Haber James, 2014).
Although high-throughput sequencing technologies have been limited in some applications due to their high error rates, repeated template sequencing presents a powerful approach for increasing the sequencing fidelity to a level that can generate highly sensitive detection assays.
It was proposed that when the replication fork stalls, e.g. due to single-strand breaks or single nucleotide deletions, repeated template switching to nearby genomic regions can occur, leading to apparent deletions when parts of the template are skipped, and to duplications when the replication machinery "jumps back".
In any case, the complex NCO resolutions seen in our pedigree data has not been reported in mammals previously, and is consistent with either with patchy GC biased repair across long stretches of heteroduplex DNA or repeated template switching during the repair of DSBs.
Alternatively, BIR initiated from one-ended DNA breaks at the sites of collapsed replication forks could be more processive, and repeated template switching (fork stalling and template switching, FoSTeS [ 131]) could result in complex genomic rearrangements and copy-number transition [ 132, 133].
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This process was repeated until template was not detected by HPLC.
We defined a repeat medicine as any medicine on a repeat template.
All practices in the PCT used SystmOne (TPP Ltd), which lists medicines authorised for repeat use on a "repeat template".
Some practices are reluctant to include potentially harmful medicines that need frequent monitoring (such as methotrexate) on the repeat template.
In particular, oral contraception is often prescribed as a six month supply without adding it to the repeat template.
Searches were conducted in November 2011 at practice population level to identify the numbers of repeat items listed on individual's repeat template.
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