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The end result of the recombination is that the stretch of DNA between the repeated sites inverts.
In order to mitigate the overfitting problem, the matrices were rebuilt eliminating repeated sites.
On DNA with two directly repeated sites, the enzymes load on to the DNA in the same direction.
Nicking reactions at many of the repeated sites along the RCA elongated single-stranded DNA occurred simultaneously.
Recombination between tandemly repeated sites would result in excision of the intervening DNA, whereas recombination between inversely oriented sites would invert the intervening DNA.
> Once the three PSSMs were obtained, they were rebuilt, excluding the repeated sites, with the aim of minimizing the problem of overfitting, and then their predictive capacity was assessed and compared in order to choose the best one.
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(E ) Structural comparison of the ANK repeats site 1 bindings of AnkR_AS and Nav1.2_ABD-C showing that the two targets bind to ANK repeats with essentially the same mode.
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