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Fortunately, interim approaches based on mtDNA differences between Neandertals and current humans, detection of male contamination through Y chromosomal sequences, and repeated sequencing from the same fossil to detect autosomal contamination allow initial large-scale sequencing of Neandertal genomes.
"So this took a lot of effort and repeated sequencing for each specimen".
Novel genetic variations were confirmed by repeated sequencing of PCR products in both directions.
The sequence of the cloned DNA was determined by dideoxy sequencing, and sequencing ambiguities were resolved by repeated sequencing on both strands.
Moreover, repeated sequencing of the same plasmid and isolated by different persons and at different time gave identical results (6 RandF1A and 6 RBSplasmidmid clones were sequenced) excluding frequent sequencing errors.
Clearly, there is a huge proportion of repeated sequencing and over representation of abundantly expressed genes.
Similar(33)
The repeated sequences delight children as they can anticipate Goldilocks audacity and revel in the mounting disapproval.
It includes adult language, repeated sequences of brilliantly staged action and brutality, and, oh, yes, alcohol consumption.
She also made her dance formally ingenious, for before it ended she repeated sequences she had introduced at the beginning.
The dancers strip to their underwear on chairs in fast repeated sequences that suggest a protest against conformity.
Repeated sequences in the pol mitotype were analyzed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com