The intervention consisted of an educational module viewed with initial audits, development of a personalized improvement plan after Phase 1, and repeated real-time feedback of individual relative performance compared at clinic and program levels.
Non-invasive whole body bioluminescence imaging (BLI) allows repeated, real-time in vivo monitoring of tumor growth in experimental animals, regardless of tumor locations.
By contrast, CTCs provide a very promising, easy to repeat, real-time "liquid biopsy" approach.
These procedures can be repeated in real time.
To confirm these results and also for a more precise evaluation of intraspecific variability of satellite DNA copy number, the quantification experiment was repeated by real time qPCR using the same P1-forward/2a-reverse primer pair.
While tissue sampling is invasive and often difficult to repeat, circulating tumor cell (CTC) analysis requires only a blood sample and might provide an easy-to-repeat, real-time "liquid biopsy" approach.
These experiments were repeated using quantitative real time RT-PCR and are shown in Fig. 5C.
Many experiments, being electronic, can be repeated quickly in real time, so failure does not result in any significant costs or time penalties, and there is a high probability that one will learn something of value in the process.
For a technical reason (insufficient specificity), the result of v-rDNA I+II was not repeated with real-time PCR (not shown).
However, given that preferential amplification of the smaller product may produce data artefacts, the study was repeated using real-time PCR (with Sybr green as the reporter dye) on the MX3005P thermal cycler (Stratagene, UK).
The whole procedure was repeated for Real-Time quantitative PCR (qRT-PCR) validation; in this case, only two biological replicas were obtained.
