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IS and internal repeated reads were then reinserted in the assembly on the clone-links basis.
4,109,921 reads found a unique match (group U of unique reads), 830,843 reads found multiple matches (group R of repeated reads), and 18,082,054 reads remained with no match (group M of missed reads).
These repeated reads were collapsed for further analysis.
Seq-dup-level (sequence duplication level) represents the proportion of repeated reads in total reads.
In addition, it is a wise precaution to filter out low-quality reads, low-complexity reads and highly repeated reads likely to result in numerous spurious alignments.
In this study, the first step of data analysis involved the first two authors' familiarisation with the data, through repeated reads of documents or interview transcripts.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com