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The Bedside Shivering Assessment Scale is a simple, validated four-point scale that enables repeated quantification of shivering at the bedside.
The method presented here permits easy, rapid, reliable and reproducible repeated quantification of epithelial cells in peripheral blood, and could serve as a tool for real-time monitoring of therapy in vivo.
Repeated quantification of CpG methylation levels at nt positions −296 and −290 (both SMN2 CGI 2) corroborated a significant correlation between CpG methylation and disease severity in type I (ML17), type IIIa (ML22) and type IIIb SMA fibroblasts (ML74) (Fig. 4A).
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In the second step of the evaluation of population tuning, we fitted sinusoids to the population tuning curves and repeated the quantification on the residuals.
Then, the protocol was repeated for quantification of actin, using a mouse anti-actin primary antibody (Millipore, Cat. no. MAB1501; dilution 1 : 700) followed by a goat anti-mouse secondary antibody (Pierce Biotechnology Inc., Cat. no. 1858413; dilution 1 : 5000).
Given the fact that a short period of adaptation is sufficient to increase the resistance to growth inhibition by SCFAs, we repeated the quantification of membrane fluidity and integrity using cells that had undergone 3 h of SCFA adaptation.
Although these programs are dedicated to TE assembly, they do not allow repeat quantification or annotation.
Genotypes were correctly detected in 60/60 samples (100%), but errors occurred in (CGG) n repeat quantification (Table 3).
However, required prerequisites for the use of 454 sequencing for repeat quantification and reconstruction are that the template sampling is random and that the sequencing does not introduce a bias towards certain sequences.
Even with similar markers, superior combined performance could be achieved by improved precision through repeated similar quantifications.
PrPres Western blot profiles from 30 mice representative of the different sheep genotypes and of the three subpopulations of TgOvPrP4 mice were subjected to repeated analyses and quantification.
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