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Its repeated motives and modularity make Rtx a multifunctional factor in pathogenicity.
Alleles type I, II and III differ only in the number and arangement of the repeated motives at the 3'end variable region of the gene as previously described in VL strains [ 10].
While long considered absent in the L. tropica group, we identified by sequencing an A2 gene from crude PCR products of two strains of L. major (AF532102, AF532103) showing that the gene is present in L. major in a truncated form lacking most of the repeated motives that are present at the 3'end variable region of the VL A2 genes.
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The Indo-Persian area was the most magical, with its repeated Islamic motives, long straight water course, and planting beds in the shape of eight pointed stars.
But it is important to understand that the revelations were not made – nor have they been repeated – for motives entirely devoted to achieving political purity.
By contrast, a major deletion of the 3'end variable region of repeated nucleotide motives was observed in L. major A2 genes.
Out of 31 repeat motives identified in the region of interest, 12 were found to be polymorphic and used for refinement of linkage analysis and haplotype reconstruction (Supplementary Tables 1 and 2).
Nine of the polymorphic SSR found in this work were located within predicted ORF and seven had repeat motives multiple of three (Table 2), according to their presence in coding regions [ 41].
The curious passages where Beethoven repeats simple motives for measures on end have seldom sounded so obsessive.
The TOX3 gene, also known as TNRC9 and CAGF9, has a tri-nucleotide repeat motive [ 27] and a high mobility group (HMG -box [ 28].
The coding region for the triple-repeat motive comprising amino acids 94 to 232 of LOX-1 protein from rat was amplified with the oligonucleotides 5'-CG GGATCCAAGAATCAAAGAGGGAACTGAA-3' (5'-end) and 5'-CCGCTCGAGACCTGAAGAGTTTG CAGCTCT-3' (3'-end), which introduced BamHI and XhoI restriction sites (underlined).
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