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The most commonly used large-animal models of ARDS include endotoxin infusion, repeated lavage, oleic acid and smoke/burn injury [17].
Repeated lavage with saline and accurate haemostasis was then performed.
This procedure mimics the applied clinical treatment of a repeated lavage.
Repeated lavage to deplete lung surfactant, followed by mechanical ventilation has been used as an experimental model of ARDS in rats [ 25].
Lung injury was performed by repeated lavage (30 ml/kg) with prewarmed 38°C sterile 0.9% saline solution through the endotracheal tube for surfactant washout.
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We observed a washout effect with decreased biomarker concentrations after repeated lavages following exposure to air.
It would be also interesting to study serially infants with repeated lavages during the ARDS course: this may give clues about the progression of inflammation.
A tracheotomy was performed in 25% urethane-anesthetized animals, by inserting a 5 mm plastic tube, and repeating lavage five times with saline and a gauze filter.
In group 2 (lavage), direct lung injury was induced by repeated bronchoalveolar lavage using saline infusion.
ARDS was obtained by repeated bronchoalveolar lavage (0.9% saline solution).
Other experimental models of acute respiratory distress syndrome (e.g., by repeated pulmonary lavage [29]) could more closely reproduce the clinical scenario.
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