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Bacterial persistence, defined as repeated isolation of an indistinguishable (by pulsed field gel electrophoresis [PFGE]) isolate at sampling times greater than 6 months, is a great concern for food industries since it can lead to the repeated contamination of food with spoilage or pathogenic microorganisms and has been demonstrated to recurrently happen for strains of L. monocytogenes [ 14].
For patients who have repeated isolation of H. influenzae despite the use of appropriate antibiotics consideration should be given to the use of longer-term antibiotics or those with good intracellular penetration.
The strong biogeographic affinities of the study area with the North Island reflect climate-related distributional limits of plant species and repeated isolation and fragmentation of populations during Pleistocene glacial-interglacial cycles; this has facilitated the disjunct distribution of many plant species (McGlone 1985).
Repeated isolation of MRSA from different clinical samples from a single patient is common; to estimate the number of infections, we determined the first clinical isolation (FCI) of MRSA for each patient admitted between 1 April 1998 and 1 July 2006, initially considering all clinical samples (excluding screens).
These findings were confirmed by repeated isolation attempts.
A diagnosis of MAC-PD requires clinical findings and its repeated isolation from sputum.
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C. immitis is highly concentrated in Native American middens, indicating that the organism was highly prevalent among these people (21 ), and repeated isolations of C. immitis have been made at old Native American campsites in endemic areas (22 ).
Another study showed that after burial of animals that died of coccidioidomycosis in soil free of the organism, repeated isolations of C. immitis from the soil at the burial site were possible for at least 7 years, even though soil immediately surrounding the burial site remained uncontaminated (20 ).
Retrieval of DNA from ancient sediments (Radax et al., 2001; Fish et al., 2002), repeated isolations from the same site (Stan-Lotter et al., 1999), and recent reports of successful haloarchaeal cultivations from well-dated bore cores (Schubert et al., 2010; Gramain et al., 2011) supported the presence of biological material in evaporites of great geological age.
However, repeated social isolation had no effect on judgment of ambiguous stimuli and on both basal and test-related cortisol levels.
The Viet Minh repeated the isolation and probing attacks against Huguette 1, and overran the fort on the morning of 22 April.
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